Chromatography is an analytical technique used for separation of components of a mixture.

In the first decade of the 20th century, this technique was primarily used for separation of plant pigments such as “Chlorophyll”.

Chromatography is a misnomer since it is no longer limited to the separation of coloured substance.

Chromatography involves distribution of a solute between two immiscible phases which are

  • Stationery Phase
  • Mobile Phase.